Microfluidic-assisted fabrication of artificial microniches for bone marrow stem cells. MICRONICHE

microniche-ue-logoThere is a growing interest in adult stem cells, especially from bone marrow, for regenerative medicine. Hematopoietic stem cells, a type of bone marrow stem cells, alone cannot be expanded in vitro; in vivo, they reside in a microenvironment known as a niche that maintains them in a quiescent state until prompted to differentiate. The stem cell niche provides structural and trophic support and the appropriate homeostasis to regulate stem cell function. Additionally to regulatory factors in these stem cell niches, a number of environmental and mechanical signals arising from the extracellular matrix are crucial regulators of stem cell fate. In order to expedite for basic studies of bone marrow stem cells, and further translational implementation, any realistic approach to the native stem cell niche requires: to engineer a biomimetic 3D-microenvironment, and then to develop artificial microniches with the key functional features reconstructed. High-throughput microfluidic technology offers high promise, however, adaptation to accommodate adult stem cells in artificially fabricated niches remains still a challenge. Microfluidic-assisted culture systems should not only allow maintaining cell homeostasis through biochemical and mechanical stimulation, but also modulating adult stem cell renewal and differentiation through microscale patterning of cells and extracellular materials in biomimetic microniches. This project aims at the microfluidic reconstruction of an artificial stem cell niches. In this proof-of-concept, a bone marrow stem cell microniche with tunable size, material and topography will be developed by integrating novel fabrication microfluidics with material engineering.
 

MicroNICHE project has been selected for publication in the ‘Results in Brief’ section of the European Commission’s CORDIS website.

Publicaciones
Revistas JCR
RESULT Microfluidics for the Ex Vivo Expansion of Human Primary Multiple Myeloma Cells
Pilar Carreras, Iciar Gonzalez, Miguel Gallardo, Alejandra Ortiz-Ruiz, Joaquin Martinez-Lopez
Micromachines 2020, 11(3)261-270
https://doi.org/10.3390/mi11030261
RESULT Influence of Hydrodynamics and Hematocrit on Ultrasound-Induced Blood Plasmapheresis
Itziar González, Roque Rubén Andrés , Alberto Pinto and Pilar Carreras
Micromachines 2020, 11, 751
https://doi.org/10.3390/mi11080751
RESULT Droplet Microfluidics for the ex Vivo Expansion of Human Primary Multiple Myeloma Cells
Pilar Carreras, Iciar Gonzalez, Miguel Gallardo, Alejandra Ortiz-Ruiz, Joaquin Martinez-Lopez
Pilar Carreras, Iciar Gonzalez, Miguel Gallardo, Alejandra Ortiz-Ruiz, Joaquin Martinez-Lopez
https://doi.org/10.3390/mi11030261